Robot-aided measurement of insect diversity on vegetation using environmental DNA

Traditional methods of biodiversity monitoring are often logistically challenging, time-consuming, require experienced experts on species identification and sometimes include destruction of the targeted specimens. Here, we investigated a non-invasive approach of combining the use of drones and environmental DNA (eDNA) to monitor insect biodiversity on vegetation. We aimed to assess the efficiency of this novel method in capturing insect diversity and comparing insect composition across different vegetation types (grassland, shrub, and forest) in Switzerland. A commercial, off-the-shelf drone was equipped with a specialised probe that autonomously swabbed vegetation and collected eDNA. Then, samples were processed using rapid third-generation Oxford Nanopore sequencing. The obtained data were analysed for insect diversity, comparing taxonomic richness, evenness and community composition across the three habitat types using statistical techniques. Sequencing of the samples yielded 76 hexapods taxa, revealing an insect community with notable differences in taxonomic richness but not in evenness across grassland, shrub, and forest habitats. Our study demonstrates the potential of drone-based sampling integrated with eDNA and nanopore sequencing for biodiversity monitoring, offering a non-destructive method for detecting insect occurrence on plant surfaces. Integrating robotics and eDNA technology provides a promising solution for fast, large-scale, non-invasive biodiversity monitoring, potentially improving conservation efforts and ecosystem management.